ARG10740

anti-Visinin like 1 antibody

anti-Visinin like 1 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Bovine,Pig

Overview

Product Description Rabbit Polyclonal antibody recognizes Visinin like 1
Tested Reactivity Hu, Ms, Rat, Bov, Pig
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Visinin like 1
Species Human
Immunogen Full length recombinant Human Visinin-like 1.
Conjugation Un-conjugated
Full Name visinin-like 1
Alternate Names HPCAL3; HUVISL1; Visinin-like protein 1; HLP3; Hippocalcin-like protein 3; VILIP; VLP-1; VILIP-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000
IHC-Fr1:2000
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 22 kDa

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 26950 Mouse VSNL1

GeneID: 282124 Bovine VSNL1

GeneID: 7447 Human VSNL1

Gene Symbol VSNL1
Gene Full Name visinin-like 1
Background This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Function Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG10740 anti-Visinin like 1 antibody ICC/IF image

    Immunocytochemistry: Rat Neuron-glia cell culture stained with ARG10740 anti-Visinin like 1 antibody (red) and co-stained with monoclonal antibody to GFAP  (green); DNA (blue). ARG10740 reveals strong staining throughout some small neurons in soma and all processes, accumulation is seen in some hotspots along neurites. GFAP appears only in astrocytes.

  • ARG10740 anti-Visinin like 1 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10740 anti-Visinin like 1 antibody (green) at 1:2000 dilution and costained with ARG10703 anti-Calretinin antibody [6A9] (red) at 1:2000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)

    The Visinin like 1 antibody reveals protein expressed in granule cells membranes and their synapses in both the granular and molecular layer of the cerebellum. The calretinin antibody stains the cytoplasm of neurons in the nuclear and molecular layers of cerebellum.

  • ARG10740 anti-Visinin like 1 antibody WB image

    Western blot: Bovine cerebellum homogenate stained with ARG10740 anti-Visinin like 1 antibody.

  • ARG10740 anti-Visinin like 1 antibody WB image

    Western blot: Rat brain, Mouse brain, Pig hippocampus and Cow cerebellum lysates stained with ARG10740 anti-Visinin like 1 antibody (green) at 1:20000 dilution.