ARG41065

anti-Vitronectin antibody

anti-Vitronectin antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Vitronectin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Vitronectin
Antigen Species Human
Immunogen Synthetic peptide derived from Human Vitronectin.
Conjugation Un-conjugated
Full Name vitronectin
Alternate Names Vitronectin; V75; VN; Serum-spreading factor; S-protein; VNT

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22370 Mouse VTN

GeneID: 7448 Human VTN

Swiss-port # P04004 Human Vitronectin

Swiss-port # P29788 Mouse Vitronectin

Gene Symbol VTN
Gene Full Name vitronectin
Background The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]
Function Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. [UniProt]
Cellular Localization Secreted, extracellular space. [UniProt]
Calculated MW 54 kDa
PTM Sulfated on 2 tyrosine residues.

N- and O-glycosylated.

Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.

It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Phosphorylation sites are present in the extracellular medium. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG41065 anti-Vitronectin antibody WB image

    Western blot: Human serum membrane lysate stained with ARG41065 anti-Vitronectin antibody.