ARG54601

anti-Vitronectin antibody [VN58-1]

anti-Vitronectin antibody [VN58-1] for Western blot,ELISA,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Overview

Product Description Mouse Monoclonal antibody [VN58-1] recognizes Vitronectin
Tested Reactivity Hu
Species Does Not React With Bov
Tested Application ELISA, ICC/IF, IHC-P, WB
Specificity This antibody specifically reacts with an epitope in the region of aa. 1- 130 of Human vitronectin. It does not react with Bovine vitronectin, and this antibody does not interfere with vitronectin-mediated adhesion.
Host Mouse
Clonality Monoclonal
Clone VN58-1
Isotype IgG1
Target Name Vitronectin
Antigen Species Human
Immunogen Human Vitronectin.
Conjugation Un-conjugated
Full Name vitronectin
Alternate Names Vitronectin; V75; VN; Serum-spreading factor; S-protein; VNT

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:3000
ICC/IFAssay-dependent
IHC-P5 - 10 µg/ml
WB5 - 10 µg/ml
Application Note WB: Under reduced and non-reduced conditions.
ELISA: With solid phase antigen.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Buffer 10 mM PBS (pH 7.4) and 1% BSA.
Stabilizer 1% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7448 Human VTN

Swiss-port # P04004 Human Vitronectin

Gene Symbol VTN
Gene Full Name vitronectin
Background The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]
Function Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. [UniProt]
Cellular Localization Secreted, extracellular space. [UniProt]
PTM Sulfated on 2 tyrosine residues.

N- and O-glycosylated.

Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.

It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Phosphorylation sites are present in the extracellular medium. [UniProt]

Clone References

Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings.

ICC/IF / Human

Zhou J et al.
Int J Nanomedicine.,  (2014)

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Interaction of late apoptotic and necrotic cells with vitronectin.

Stepanek O et al.
PLoS One.,  (2011)

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Differential affinity of vitronectin versus collagen for synthetic biodegradable scaffolds for urethroplastic applications.

IHC-P / Human

Hicks BG et al.
Biomaterials.,  (2011)

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A Toll-like receptor 2-integrin beta3 complex senses bacterial lipopeptides via vitronectin.

Gerold G et al.
Nat Immunol.,  (2008)

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