ARG54144

anti-WDR77 antibody

anti-WDR77 antibody for Western blot,ICC/IF and Human,Mouse,Rat

Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody recognizes MEP50/WDR77
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name WDR77
Antigen Species Human
Immunogen Purified recombinant human WDR77 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names Nbla10071; p44/Mep50; WD repeat-containing protein 77; MEP50; HKMT1069; Methylosome protein 50; p44; MEP-50; Androgen receptor cofactor p44

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 42 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 310769 Rat WDR77

GeneID: 70465 Mouse WDR77

GeneID: 79084 Human WDR77

Gene Symbol WDR77
Gene Full Name WD repeat domain 77
Background Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Function Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. [UniProt]
Cellular Localization Nucleus. Cytoplasm.
Research Area Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 37 kDa

Images (2) Click the Picture to Zoom In

  • ARG54144 anti-WDR77 antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed in 1% Paraformaldehyde, permeabilized in 0.1% Triton X-100 and stained with ARG54144 anti-WDR77 antibody at 1:100 dilution.

  • ARG54144 anti-WDR77 antibody WB image

    Western blot: K562 cell lysate stained with ARG54144 anti-WDR77 antibody at 1:1000 dilution.