ARG46658

anti-ZBED1 antibody

anti-ZBED1 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes ZBED1
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ZBED1
Antigen Species Human
Immunogen A 15 amino acid synthetic peptide within the last 50 amino acids of human ZBED1.
Conjugation Un-conjugated
Alternate Names zinc finger, BED-type containing 1; ZBED1; ALTE; DREF; TRAMP; hDREF; ALTE; KIAA0785; Zinc finger BED domain-containing protein 1; Putative Ac-like transposable element

Application Instructions

Application Suggestion
Tested Application Dilution
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -708°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9189 Human ZBED1

Swiss-port # O96006 Human Zinc finger BED domain-containing protein 1

Gene Symbol ZBED1
Gene Full Name zinc finger, BED-type containing 1
Background This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. [provided by RefSeq, Jan 2010]
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA (PubMed:27068747). This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12 (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747). The resulting increased transcriptional activity drives cell proliferation (PubMed:12663651, PubMed:17220279). Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747). [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 78 kDa
PTM Ubl conjugation. [UniProt]