ARG57121
anti-ZNF9 / CNBP antibody [38F10]
anti-ZNF9 / CNBP antibody [38F10] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [38F10] recognizes ZNF9 / CNBP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 38F10 |
| Isotype | IgG2b, kappa |
| Target Name | ZNF9 / CNBP |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-170 of Human ZNF9 / CNBP |
| Conjugation | Un-conjugated |
| Alternate Names | RNF163; ZCCHC22; PROMM; CNBP1; ZNF9; Zinc finger protein 9; CNBP; DM2; Cellular nucleic acid-binding protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P62633 Human Cellular nucleic acid-binding protein |
|---|---|
| Gene Symbol | CNBP |
| Gene Full Name | CCHC-type zinc finger, nucleic acid binding protein |
| Background | This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Single-stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. [UniProt] |
| Calculated MW | 19 kDa |
| PTM | Arginine methylation by PRMT1 in the Arg/Gly-rich region impedes RNA binding. |
Images (4) Click the Picture to Zoom In
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] FACS image
Flow Cytometry: MCF7 cell line stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] WB image
Western blot: 30 µg of 293T cell lysate stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 1) 1:250, 2) 1:500, and 3) 1:1000.
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] ICC/IF image
Immunofluorescence: MCF7 cells line stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
