ARG65781

anti-Zika virus NS1 antibody [SQab1609]

anti-Zika virus NS1 antibody [SQab1609] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Virus

publication_link Publication5

Overview

Product Description

Mouse Monoclonal antibody [SQab1609] recognizes Zika virus NS1

Tested Reactivity Virus
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity This Zika virus NS1 antibody specifically reacts to Zika virus NS1 protein and do not cross-react to DENV and CHIKV tested by ELISA. And a paper published by Bollweg BC et al, 2017 also validated that this antibody do not cross-react to other flaviviruses and chikungunya virus by IHC-P samples.
Host Mouse
Clonality Monoclonal
Clone SQab1609
Isotype IgG1
Target Name Zika virus NS1
Antigen Species Virus
Immunogen Zika virus NS1
Conjugation Un-conjugated
Full Name Zika virus nonstructural protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:3000 - 1:10000
FACS1:400 - 1:1000
ICC/IF1:200 - 1:1000
IHC-FrAssay-dependent
IHC-PAssay-dependent
WB1:1000 - 1:5000
Application Note ELISA: The antibody can be used as capture antibody in sandwich ELISA for Zika virus NS1 detection in combination with ARG65783 anti-Zika virus NS1 antibody [SQab1610] (Biotin) as detection antibody.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS (pH 7.4) and 0.01% Thimerosal.
Preservative 0.01% Thimerosal.
Concentration 1.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Zika virus nonstructural protein 1
Background The mosquito-borne Zika virus (ZIKV) is prompting worldwide concern due to its connection to neurological disorders including microcephaly. Zika virus belongs to the flavivirus family which encompasses Dengue, yellow fever, West Nile and more different viruses. The connection of neurological disorders creates a need for further research into the ZIKV infection and therapeutic approaches.
For more ZIKA virus antibody and ZIKA virus antibody Duo products, please refer to Choose the Best ZIKA Virus Antibodies
Highlight Related Antibody Duos and Panels:
ARG30277 Zika virus NS1 ELISA Duo
Related products:
Zika virus antibodies; Zika virus Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Choose the Best ZIKA Virus Antibodies
Best Zika Virus antibodies cited by CDC & A*STAR
Exploring Antiviral Immune Response

Images (7) Click the Picture to Zoom In

  • ARG65781 anti-Zika virus NS1 antibody [SQab1609] ICC/IF image

    Immunofluorescence: ARG65781 anti-Zika virus NS1 antibody [SQab1609] (1:400) were used for detecting ZIKV NS1.

  • ARG65781 anti-Zika virus NS1 antibody [SQab1609] FACS image

    Flow Cytometry: Zika virus NS1 expressing plasmid transfected (red) or non-transfected (blue) 293T cells stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:1000 dilution.

  • ARG65781 anti-Zika virus NS1 antibody [SQab1609] WB image

    Western blot: 20 μg of 293T cell lysates transfected with 1) control plasmid or 2) Zika virus NS1 expression plasmid. The blots were stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:5000 dilution.

  • ARG65781 anti-Zika virus NS1 antibody [SQab1609] WB image

    Wetern blot: 100 ng of 1) ZIKV NS1 (Purified from E.coli), 2) DENV1 NS1 (Purified from 293 cell), 3) DENV2 NS1 (Purified from 293 cell), 4) DENV3 NS1 (Purified from 293 cell), 5) DENV4 NS1 (Purified from 293 cell), and 6) CHIKV NS1 (Purified from 293 cell) stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:5000 dilution.

  • ARG65781 anti-Zika virus NS1 antibody [SQab1609] WB image

    Western blot: 10 μg of Vero cells Infected with 1) Mock, 2) ZIKV, 3) DENV1, 4) DENV2, 5) DENV3 and 6) DENV 4. Cell lysates were stained with ARG65781 anti-Zika virus NS1 antibody [SQab1609] at 1:2000.

  • ARG65781 anti-Zika virus NS1 antibody [SQab1609] & ARG65783 anti-Zika virus NS1 antibody [SQab1610] (Biotin) ELISA image

    ELISA: 293 cells expressed DENV1-4, CHIKV NS1 protein and E.coli expressed ZIKV NS1 protein detected by ARG65781 anti-Zika virus NS1 antibody [SQab1609] as capture antibody (5 µg/ml), and ARG65783 anti-Zika virus NS1 antibody [SQab1610] (Biotin) (1 µg/ml) as detection antibody, followed by incubation with streptavidin-HRP.

  • ARG65781 anti-Zika virus NS1 antibody [SQab1609] ELISA image

    ELISA: ARG65781 anti-Zika virus NS1 antibody [SQab1609] (1:5000) were used for detecting ZIKV NS1. Each protein (1 µg/ml) were coated onto ELISA plate.

Specific References

Zika Virus Infects Human Placental Mast Cells and the HMC-1 Cell Line, and Triggers Degranulation, Cytokine Release and Ultrastructural Changes.

ICC/IF, IHC-P, FACS / Zika virus

Rabelo Kissila et al.
Cells.,  (2020)

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Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil.

IHC-P / Zika virus

Almeida, Paula Rodrigues et al.
Preprint.,  (2019)

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Monodelphis domestica as a fetal intra-cerebral inoculation model for Zika virus pathogenesis

IHC-Fr / Zika virus

John M. Thomas III et al.
Preprint.,  (2019)

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Placental Histopathology and Clinical Presentation of Severe Congenital Zika Syndrome in a Human Immunodeficiency Virus-Exposed Uninfected Infant.

IHC-P / Zika virus

Rabelo K et al.
Front Immunol.,  (2017)

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Optimization of Commercially Available Zika Virus Antibodies for Use in a Laboratory Developed Immunohistochemical Assay

IHC-P / Zika virus

Brigid C. Bollweg et al.
The Journal of Pathology: Clinical Research.,  (2017)

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