ARG10363

anti-alpha Fetoprotein antibody [4A3]

anti-alpha Fetoprotein antibody [4A3] for ELISA and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody

Overview

Product Description Mouse Monoclonal antibody [4A3] recognizes alpha Fetoprotein
Tested Reactivity Hu
Tested Application ELISA
Host Mouse
Clonality Monoclonal
Clone 4A3
Isotype IgG2a
Target Name alpha Fetoprotein
Antigen Species Human
Immunogen purified human alpha-fetoprotein.
Conjugation Un-conjugated
Full Name alpha-fetoprotein
Alternate Names Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 174 Human AFP

Swiss-port # P02771 Human Alpha-fetoprotein

Gene Symbol AFP
Background This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt]
Resrarch Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
PTM Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

Clone References

Three-dimensional perfusion bioreactor culture supports differentiation of human fetal liver cells.

sELISA / Human

Schmelzer E et al.
Tissue Eng Part A.,  (2010)

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Free antibody sample