ARG51766

anti-alpha Synuclein phospho (Tyr136) antibody

anti-alpha Synuclein phospho (Tyr136) antibody for Western blot,ICC/IF and Human,Mouse,Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes alpha Synuclein phospho (Tyr136)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name alpha Synuclein
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine 136 (Q-D-Y(p)-E-P) derived from Human α-Synuclein.
Conjugation Un-conjugated
Alternate Names Non-A4 component of amyloid precursor; Alpha-synuclein; PARK4; PARK1; PD1; NACP; Non-A beta component of AD amyloid

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20617 Mouse SNCA

GeneID: 29219 Rat SNCA

GeneID: 6622 Human SNCA

Gene Symbol SNCA
Gene Full Name synuclein, alpha (non A4 component of amyloid precursor)
Background May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Function May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. [UniProt]
Research Area Neuroscience antibody
Calculated MW 14 kDa
PTM Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form (By similarity).
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.

Images (2) Click the Picture to Zoom In

  • ARG51766 anti-alpha Synuclein phospho (Tyr136) antibody WB image

    Western blot: Extracts from Mouse brain tissue stained with ARG51766 anti-alpha Synuclein phospho (Tyr136) antibody and the same antibody preincubated with blocking peptide.

  • ARG51766 anti-alpha Synuclein phospho (Tyr136) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51766 anti-alpha Synuclein phospho (Tyr136) antibody.