ARG53749

anti-beta 2 Microglobulin antibody [B2M-01] (HRP)

anti-beta 2 Microglobulin antibody [B2M-01] (HRP) for ICC/IF,Western blot,ELISA and Human

Cancer antibody; Cell Biology and Cellular Response antibody

Overview

Product Description HRP-conjugated Mouse Monoclonal antibody [B2M-01] recognizes beta 2 Microglobulin
Tested Reactivity Hu
Species Does Not React With Ms, Bov, Chk, Dog, Rb
Tested Application ELISA, ICC/IF, WB
Specificity The clone B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes._x000D_
Host Mouse
Clonality Monoclonal
Clone B2M-01
Isotype IgG2a
Target Name beta 2 Microglobulin
Antigen Species Human
Immunogen Purified human beta2-microglobulin
Conjugation HRP
Alternate Names Beta-2-microglobulin

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with horseradish peroxidase (HRP) of high specific activity and RZ=3.
Buffer PBS, 0.01% (w/v) Thimerosal and 1% (w/v) high-grade protease free BSA
Preservative 0.01% (w/v) Thimerosal
Stabilizer 1% (w/v) high-grade protease free BSA
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 567 Human B2M

Swiss-port # P61769 Human Beta-2-microglobulin

Gene Symbol B2M
Gene Full Name beta-2-microglobulin
Background Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody
Calculated MW 14 kDa
PTM Glycation of Ile-21 is observed in long-term hemodialysis patients.

Images (2) Click the Picture to Zoom In

  • ARG53749 anti-beta 2 Microglobulin antibody [B2M-01] (HRP) ELISA image

    ELISA: HLA-G in human serum using anti-HLA-G antibody (capture) and ARG53749 anti-beta 2 Microglobulin antibody [B2M-01] (HRP) (detection).

  • ARG53749 anti-beta 2 Microglobulin antibody [B2M-01] (HRP) ELISA image

    ELISA: Analysis of HLA-G and beta 2 Microglobulin in supernatant of HLA-G transfectants with ARG53749 anti-beta 2 Microglobulin antibody [B2M-01] (HRP) as the detection antibody.

Clone References

Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.

Tiwari S et al.
J Cell Sci.,  (2011)

publication_link

 

hr_line

FcRn binding properties of an abnormal truncated analbuminemic albumin variant.

ELISA / Human

Andersen JT et al.
Clin Biochem.,  (2010)

publication_link

 

hr_line

Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B.

WB / Human

Slany A et al.
J Proteome Res.,  (2010)

publication_link

 

hr_line

Oncostatin M enhances the antiviral effects of type I interferon and activates immunostimulatory functions in liver epithelial cells.

WB / Human

Larrea E et al.
J Virol.,  (2009)

publication_link

 

hr_line