ARG10206

anti-beta 2 Microglobulin antibody [D2E9]

anti-beta 2 Microglobulin antibody [D2E9] for ELISA,Western blot,Flow cytometry,ICC/IF and Human

Cancer antibody; Cell Biology and Cellular Response antibody

Overview

Product Description Mouse Monoclonal antibody [D2E9] recognizes Human beta 2 microglobulin
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone D2E9
Isotype IgG1,k
Target Name beta 2 Microglobulin
Antigen Species Human
Immunogen Highly purified beta 2 microglobulin from human urine
Conjugation Un-conjugated
Full Name beta-2-microglobulin
Alternate Names Beta-2-microglobulin

Application Instructions

Application Note ELISA: Clone D2E9 can be used as capturer in Sandwich ELISA for B2MG detection.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 567 Human B2M

Swiss-port # P61769 Human Beta-2-microglobulin

Gene Symbol B2M
Gene Full Name beta-2-microglobulin
Background Human beta 2 microglobulin (B2MG) is a component of major histocompatibility complex 1 (MHC1). It is abundant at the surface of lymphocytes and exists at the surface of all cell types except red cells and platelets. B2MG is normally filtered out of blood through glomeruli, but the process is impaired in patients with renal diseases. Elevated B2MG level is implicated with lymphoma, multiple myeloma, renal diseases, viral infections and auto-immune conditions.
Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody
PTM Glycation of Ile-21 is observed in long-term hemodialysis patients.

(3) Click the Picture to Zoom In

  • ARG10206 anti-beta 2 Microglobulin antibody [D2E9] WB image

    Western Blot: 1. B2MG antigen (100 ng) 2. Jurkat cell lysate stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) at 1µg/mL

    The molecular weight of B2MG is about 13-14KD

  • ARG10206 anti-beta 2 Microglobulin antibody [D2E9] Flow Cytometry image

    Flow Cytometry: HeLa cells stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) followed by incubation with fluorescence labelled secondary antibody.

  • ARG10206 anti-beta 2 Microglobulin antibody [D2E9] ICC/IF image

    Immunofluorescence: Methanol-fixed HeLa cells stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) (green)