ARG46782
anti-beta Actin antibody
anti-beta Actin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes beta Actin |
|---|---|
| Tested Reactivity | Hu, Ms, Mk |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Detects a band of approximately 42 kDa by Western blot in the following monkey (COS-7) whole cell lysates. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | beta Actin |
| Antigen Species | Human |
| Immunogen | Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human beta Actin produced in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 42 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Epitope affinity purified |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACTB |
| Gene Full Name | actin, beta |
| Background | Beta actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017] |
| Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). [UniProt] |
| Calculated MW | 42 kDa |
| PTM | ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
Images (9) Click the Picture to Zoom In
-
ARG46782 anti-beta Actin antibody IHC-P image
Immunohistochemistry: Human appendix stained with ARG46782 anti-beta Actin antibody at 1:500 dilution.
-
ARG46782 anti-beta Actin antibody IHC-P image
Immunohistochemistry: Human lymph node stained with ARG46782 anti-beta Actin antibody at 1:500 dilution.
-
ARG46782 anti-beta Actin antibody IHC-P image
Immunohistochemistry: Mouse kidney stained with ARG46782 anti-beta Actin antibody at 1:500 dilution.
-
ARG46782 anti-beta Actin antibody IHC-P image
Immunohistochemistry: Mouse liver stained with ARG46782 anti-beta Actin antibody at 1:500 dilution.
-
ARG46782 anti-beta Actin antibody ICC/IF image
Immunofluorescence: NHI/3T3 stained with ARG46782 anti-beta Actin antibody at 1:100 dilution.
-
ARG46782 anti-beta Actin antibody ICC/IF image
Immunofluorescence: NHI/3T3 stained with ARG46782 anti-beta Actin antibody at 1:100 dilution.
-
ARG46782 anti-beta Actin antibody ICC/IF image
Immunofluorescence: NHI/3T3 stained with ARG46782 anti-beta Actin antibody at 1:100 dilution.
-
ARG46782 anti-beta Actin antibody IHC-P image
Immunohistochemistry: Human Mouse heart stained with ARG46782 anti-beta Actin antibody at 1:500 dilution.
-
ARG46782 anti-beta Actin antibody WB image
Western blot: COS-7 stained with ARG46782 anti-beta Actin antibody at 1:500 dilution.
