ARG46780

anti-beta Catenin antibody

anti-beta Catenin antibody for Western blot,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Goat Polyclonal antibody recognizes beta Catenin
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name beta Catenin
Antigen Species Human
Immunogen Purified recombinant peptide derived from within residues 731 aa to the C-terminus of human beta Catenin produced in E. coli.
Conjugation Un-conjugated
Alternate Names CTNNB; Armadillo; MRD19; Beta-Catenin; Cadherin-Associated Protein; EVR7

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50-1:250
IHC-P1:250 - 1:1,000
WB1:500 - 1:2,000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Epitope affinity purified
Buffer PBS, 20% Glycerol and 0.05% Sodium Azide
Preservative 0.05% Sodium Azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12387 Mouse CTNNB1

GeneID: 1499 Human CTNNB1

Swiss-port # P35222 Human Catenin beta-1

Swiss-port # Q02248 Mouse Catenin beta-1

Gene Symbol CTNNB1
Gene Full Name Catenin (cadherin-associated protein), beta 1
Background The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a destruction complex with AXIN1/2, APC, CSNK1A1 and GSK3B that phosphorylates and ubiquitinates CTNNB1 for proteasomal degradation. With Wnt signaling, CTNNB1 accumulates in the nucleus and acts as a coactivator for TCF/LEF transcription factors to activate Wnt-responsive genes. Also regulates cell-cell adhesion as part of the E-cadherin:catenin complex, and participates in centrosome cohesion, insulin internalization, and neurogenesis. [UniProt]
Cellular Localization Cell junction; Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane; Nucleus; Synapse. [UniProt]
Calculated MW 85.5 kDa
PTM Acetylation; Glycoprotein; Phosphoprotein; S-nitrosylation; Ubl conjugation. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG46780 anti-beta Catenin antibody ICC/IF image

    Immunofluorescence: BEAS-2B stained with ARG46780 anti-beta Catenin antibody.

  • ARG46780 anti-beta Catenin antibody WB image

    Western blot: MNT1 stained with ARG46780 anti-beta Catenin antibody.

  • ARG46780 anti-beta Catenin antibody IHC-P image

    Immunohistochemistry: Mouse intestine stained with ARG46780 anti-beta Catenin antibody.

  • ARG46780 anti-beta Catenin antibody IHC-P image

    Immunohistochemistry: Mouse liver stained with ARG46780 anti-beta Catenin antibody.

  • ARG46780 anti-beta Catenin antibody IHC-P image

    Immunohistochemistry: Mouse lung stained with ARG46780 anti-beta Catenin antibody.