ARG52651

anti-beta Catenin antibody

anti-beta Catenin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes beta Catenin

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name beta Catenin
Antigen Species Human
Immunogen Synthetic peptide from C-terminus (768-781) of the beta catenin protein.
Conjugation Un-conjugated
Alternate Names CTNNB; armadillo; MRD19; Catenin beta-1; Beta-catenin

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:300
IHC-FrAssay-dependent
IHC-P1:100 - 1:300
IP5 µg/ml
WB1:200 - 1:1000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Breast Carcinoma, A431

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12387 Mouse CTNNB1

GeneID: 1499 Human CTNNB1

GeneID: 84353 Rat CTNNB1

Background The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. β-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between α-actinin and α-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
Cellular Localization Cytoplasm, Membrane
Highlight Related Antibody Duos and Panels:
ARG30144 Phospho beta Catenin Antibody Panel (Total, pS33, pS37, pT41/pS45)
Related products:
beta Catenin antibodies; beta Catenin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Besides tumor suppression, what’s p53 busy for during embryogenesis?
Wnt / beta-catenin signaling for gastric fundus specification
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 85 kDa
PTM Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:25169422). Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation (By similarity).
S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions.
O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1.
Deacetylated at Lys-49 by SIRT1.

Images (2) Click the Picture to Zoom In

  • ARG52651 anti-beta Catenin antibody WB image

    Western blot: 30 µg of 1) 293T, 2) 3T3, and 3) Mouse liver lysate stained with ARG52651 anti-beta Catenin antibody at 1:500 dilution.

  • ARG52651 anti-Catenin-beta antibody IHC-P image

    Immunohistochemistry: Human Breast Carcinoma stained with Catenin-beta antibody (ARG52651)

Customer's Feedback

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Review for anti-beta Catenin antibody

Application:WB

Sample:Rat liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Diuretic Action of Apelin-13 Mediated by Inhibiting cAMP/PKA/sPRR Pathway.

WB / Mouse

Yanting Chen et al.
Front. Physiol.,  (2021)

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Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.

WB / Human

Jie Lian et al.
J Cancer.,  (2020)

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Narasin inhibits tumor metastasis and growth of ERα-positive breast cancer cells by inactivation of the TGF-β/SMAD3 and IL-6/STAT3 signaling pathways.

WB / Human

Jing Chen et al.
Molecular Medicine Reports.,  (2020)

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