anti-beta Catenin antibody
anti-beta Catenin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Rabbit Polyclonal antibody recognizes beta Catenin
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-Fr, IHC-P, IP, WB|
|Target Name||beta Catenin|
|Immunogen||Synthetic peptide from C-terminus (768-781) of the beta catenin protein.|
|Alternate Names||CTNNB; armadillo; MRD19; Catenin beta-1; Beta-catenin|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||Breast Carcinoma, A431|
|Purification||Immunogen affinity purified|
|Buffer||PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide|
|Preservative||< 0.1% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. β-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between α-actinin and α-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.|
|Cellular Localization||Cytoplasm, Membrane|
|Highlight||Related Antibody Duos and Panels:
ARG30144 Phospho beta Catenin Antibody Panel (Total, pS33, pS37, pT41/pS45)
beta Catenin antibodies; beta Catenin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Wnt / beta-catenin signaling for gastric fundus specification
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody|
|Calculated MW||85 kDa|
|PTM||Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:25169422). Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation (By similarity).
S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions.
O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1.
Deacetylated at Lys-49 by SIRT1.
Images (2) Click the Picture to Zoom In
ARG52651 anti-beta Catenin antibody WB image
Western blot: 30 µg of 1) 293T, 2) 3T3, and 3) Mouse liver lysate stained with ARG52651 anti-beta Catenin antibody at 1:500 dilution.
ARG52651 anti-Catenin-beta antibody IHC-P image
Immunohistochemistry: Human Breast Carcinoma stained with Catenin-beta antibody (ARG52651)