ARG51507

anti-c-Jun phospho (Ser73) antibody

anti-c-Jun phospho (Ser73) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes c-Jun phospho (Ser73)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name c-Jun
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from Human c-Jun.
Conjugation Un-conjugated
Alternate Names AP1; AP-1; Transcription factor AP-1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; Activator protein 1; c-Jun

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16476 Mouse JUN

GeneID: 24516 Rat JUN

GeneID: 3725 Human JUN

Gene Symbol JUN
Gene Full Name jun proto-oncogene
Background Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30225 Phospho c-Jun Antibody Duo (Total, pS73)
Related products:
c-Jun antibodies; c-Jun Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody
Calculated MW 36 kDa
PTM Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.

Images (3) Click the Picture to Zoom In

  • ARG51507 anti-c-Jun phospho (Ser73) antibody WB image

    Western blot: Extracts from PC14 cells untreated or treated with EGF stained with ARG51507 anti-c-Jun phospho (Ser73) antibody.

  • ARG51507 anti-c-Jun phospho (Ser73) antibody WB image

    Western blot: Extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), stained with ARG51507 anti-c-Jun phospho (Ser73) antibody.

  • ARG51507 anti-c-Jun phospho (Ser73) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained ARG51507 anti-c-Jun phospho (Ser73) antibody (left) or the same antibody preincubated with blocking peptide (right).