anti-c-Jun phospho (Thr239) antibody
anti-c-Jun phospho (Thr239) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody
|Product Description||Rabbit Polyclonal antibody recognizes c-Jun phospho (Thr239)|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-P, WB|
|Immunogen||Peptide sequence around phosphorylation site of threonine 239 (G-E-T(p)-P-P) derived from Human c-Jun.|
|Alternate Names||AP1; AP-1; Transcription factor AP-1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; Activator protein 1; c-Jun|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.|
|Buffer||PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||jun proto-oncogene|
|Background||Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.|
|Function||Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. [UniProt]|
|Research Area||Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody|
|Calculated MW||36 kDa|
|PTM||Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.
Images (3) Click the Picture to Zoom In
ARG51528 anti-c-Jun phospho (Thr239) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with UV stained with ARG51528 anti-c-Jun phospho (Thr239) antibody.
ARG51528 anti-c-Jun phospho (Thr239) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51528 anti-c-Jun phospho (Thr239) antibody (left) or the same antibody preincubated with blocking peptide (right).
ARG51528 anti-c-Jun phospho (Thr239) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51528 anti-c-Jun phospho (Thr239) antibody.