anti-cGAS antibody

anti-cGAS antibody for ICC/IF,Western blot and Human,Mouse,Rat

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Product Description Rabbit Polyclonal antibody recognizes cGAS
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name cGAS
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 350-522 of Human MB21D1 (NP_612450.2).
Conjugation Un-conjugated
Alternate Names cGAMP synthase; Cyclic GMP-AMP synthase; Mab-21 domain-containing protein 1; C6orf150; EC; cGAS; h-cGAS

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat heart, Mouse heart and MCF7


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 115004 Human MB21D1

GeneID: 214763 Mouse MB21D1

Swiss-port # Q8C6L5 Mouse Cyclic GMP-AMP synthase

Swiss-port # Q8N884 Human Cyclic GMP-AMP synthase

Gene Symbol MB21D1
Gene Full Name Mab-21 domain containing 1
Function Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. [UniProt]
Cellular Localization Cytoplasm, cytosol. Note=Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1) (PubMed:26048138). Note=(Microbial infection) Upon infection with virulent M.tuberculosis forms aggregates with dsDNA, non-virulent bacteria (without the ESX-1 locus) do not form these aggregates (PubMed:26048138). [UniProt]
Highlight Related products:
cGAS antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Calculated MW 59 kDa
PTM Polyglutamylated by TTLL6 at Glu-286, leading to impair DNA-binding activity. Monoglutamylated at Glu-314 by TTLL4, leading to impair the nucleotidyltransferase activity. Deglutamylated by AGBL5/CCP5 and AGBL6/CCP6.

Cleaved by CASP1 at Asp-140 and Asp-157 upon DNA virus infection; the cleavage impairs cGAMP production (PubMed:28314590). Also cleaved by the pyroptotic CASP4 and CASP5 during non-canonical inflammasome activation; they don't cut at the same sites than CASP1 (PubMed:28314590). [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG40192 anti-cGAS antibody WB image (Customer's Feedback)

    Western blot: Mouse heart and Rat heart tissue stained with ARG40192 anti-cGAS antibody at 1:1000 dilution for overnight at 4ºC.

  • ARG40192 anti-cGAS antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells stained with ARG40192 anti-cGAS antibody at 1:100 dilution.

  • ARG40192 anti-cGAS antibody WB image

    Western blot: 25 µg of Rat heart, Mouse heart and MCF7 cell lysates stained with ARG40192 anti-cGAS antibody at 1:1000 dilution.

Customer's Feedback

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Sample:Mouse heart, Rat heart

Sample Loading Amount:20 μg

SDS Page Type:Denatured

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP

Specific References

Growth-promoting function of the cGAS-STING pathway in triple-negative breast cancer cells

WB (shRNA varified) / Human

Liang-Chih Liu et al.
Front Oncol.,  (2022)




Evading immune surveillance via tyrosine phosphorylation of nuclear PCNA

WB / Mouse

Yuan-Liang Wang et al.
Cell Rep.,  (2021)