anti-cGAS antibody

anti-cGAS antibody for Flow cytometry,Western blot and Human

Cell Biology and Cellular Response antibody
publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes cGAS
Tested Reactivity Hu
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name cGAS
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 266-295 (Center) of Human cGAS.
Conjugation Un-conjugated
Alternate Names cGAMP synthase; Cyclic GMP-AMP synthase; Mab-21 domain-containing protein 1; C6orf150; EC; cGAS; h-cGAS

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7


Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 115004 Human MB21D1

Swiss-port # Q8N884 Human Cyclic GMP-AMP synthase

Gene Symbol MB21D1
Gene Full Name Mab-21 domain containing 1
Function Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. [UniProt]
Cellular Localization Cytoplasm, cytosol
Highlight Related products:
cGAS antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area Cell Biology and Cellular Response antibody
Calculated MW 59 kDa
PTM Polyglutamylated by TTLL6 at Glu-286, leading to impair DNA-binding activity. Monoglutamylated at Glu-314 by TTLL4, leading to impair the nucleotidyltransferase activity. Deglutamylated by AGBL5/CCP5 and AGBL6/CCP6.
Cleaved by CASP1 at Asp-140 and Asp-157 upon DNA virus infection; the cleavage impairs cGAMP production (PubMed:28314590). Also cleaved by the pyroptotic CASP4 and CASP5 during non-canonical inflammasome activation; they don't cut at the same sites than CASP1 (PubMed:28314590).

Images (1) Click the Picture to Zoom In

  • ARG54961 anti-cGAS antibody WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG54961 anti-cGAS antibody at 1:1000 dilution.

Specific References

The DNA sensor cGAS is decorated by acetylation and phosphorylation modifications in the context of immune signaling.

WB / Human

Song Bokai et al.
Mol Cell Proteomics.,  (2020)