ARG51760

anti-eIF2 alpha phospho (Ser51) antibody

anti-eIF2 alpha phospho (Ser51) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Gene Regulation antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes eIF2 alpha phospho (Ser51)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name eIF2 alpha
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2α.
Conjugation Un-conjugated
Alternate Names eIF-2alpha; EIF-2A; Eukaryotic translation initiation factor 2 subunit 1; EIF2; EIF-2alpha; EIF2A; eIF-2-alpha; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2A; EIF-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13665 Mouse EIF2S1

GeneID: 1965 Human EIF2S1

GeneID: 54318 Rat EIF2S1

Gene Symbol EIF2S1
Gene Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Background Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Function Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 36 kDa
PTM Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity).

Images (4) Click the Picture to Zoom In

  • ARG51760 anti-eIF2 alpha phospho (Ser51) antibody WB image

    Western blot: Extracts from Jurkat cells untreated or treated with H2O2 stained with ARG51760 anti-eIF2 alpha phospho (Ser51) antibody.

  • ARG51760 anti-eIF2 alpha phospho (Ser51) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51760 anti-eIF2 alpha phospho (Ser51) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51760 anti-eIF2 alpha phospho (Ser51) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51760 anti-eIF2 alpha phospho (Ser51) antibody.

  • ARG51760 anti-eIF2 alpha phospho (Ser51) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue, stained with ARG51760 anti-eIF2 alpha phospho (Ser51) antibody.

Specific References

Mesenchymal stem cells enhance autophagy of human intrahepatic biliary epithelial cells in vitro.

WB / Human

Zhu Yun et al.
Cell Biochem Funct.,  (2018)

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