ARG51814

anti-eIF4B phospho (Ser422) antibody

anti-eIF4B phospho (Ser422) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes eIF4B phospho (Ser422)
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name eIF4B
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B.
Conjugation Un-conjugated
Alternate Names EIF-4B; eIF-4B; PRO1843; Eukaryotic translation initiation factor 4B

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1975 Human EIF4B

GeneID: 75705 Mouse EIF4B

Swiss-port # P23588 Human Eukaryotic translation initiation factor 4B

Swiss-port # Q8BGD9 Mouse Eukaryotic translation initiation factor 4B

Gene Symbol EIF4B
Gene Full Name eukaryotic translation initiation factor 4B
Background Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 69 kDa
PTM Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Images (2) Click the Picture to Zoom In

  • ARG51814 anti-eIF4B phospho (Ser422) antibody WB image

    Western blot: Extracts from HeLa cells untreated or treated with EGF stained with ARG51814 anti-eIF4B phospho (Ser422) antibody.

  • ARG51814 anti-eIF4B phospho (Ser422) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinomatissue stained with ARG51814 anti-eIF4B phospho (Ser422) antibody (left) or the same antibody preincubated with blocking peptide (right).