ARG20524

anti-eNOS phospho (Ser1177) antibody

anti-eNOS phospho (Ser1177) antibody for ELISA,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes eNOS phospho (Ser1177)
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, WB
Specificity The antibody detects a ~140 kDa band corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells grown normally or treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name eNOS
Antigen Species Human
Immunogen Synthetic peptide (coupled to carrier protein) around Ser1177 of Human eNOS. This sequence is conserved in rat and mouse eNOS.
Conjugation Un-conjugated
Full Name nitric oxide synthase 3 (endothelial cell)
Alternate Names Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 133 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 1 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18127 Mouse NOS3

GeneID: 24600 Rat NOS3

GeneID: 4846 Human NOS3

Gene Symbol NOS3
Gene Full Name nitric oxide synthase 3 (endothelial cell)
Background Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes, inducible NOS (iNOS, NOS-II), neuronal NOS (bNOS, NOS-I), and endothelial NOS (eNOS, ecNOS, NOS-III). Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation. Tyr-657 is phosphorylated by PYK2 in response to fluid shear stress and this phosphorylation leads to attenuation of enzyme activity.
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30247 Phospho eNOS Antibody Duo (Total, pY1177)
Related products:
eNOS antibodies; eNOS ELISA Kits; eNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
PTM Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

Images (1) Click the Picture to Zoom In

  • ARG20524 anti-eNOS phospho (Ser1177) antibody WB image

    Western blot: 1) and 2) calyculin A (100 nM) treated Human umbilical vein endothelial cells for 30 min, 2) then the blots were treated with lambda phosphatase. The blots were stained with ARG20524 anti-eNOS phospho (Ser1177) antibody.

Specific References

Isolation and Characterization of Three Anti-hypertension Peptides from the Mycelia of Ganoderma Lucidum (Agaricomycetes).

WB / Human

Wu Qiang et al.
J Agric Food Chem.,  (2019)

publication_link

 

hr_line