ARG20525

anti-eNOS phospho (Ser632) antibody [M232]

anti-eNOS phospho (Ser632) antibody [M232] for ELISA,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody [M232] recognizes eNOS phospho (Ser632)
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, WB
Specificity The antibody detects a ~140 and 120 kDa bands on SDS-PAGE immunoblots of human umbilical vein endothelial cells, but these bands are not observed after lambda phosphatase treatment. The 120 kDa band may be a truncated form of eNOS.
Host Mouse
Clonality Monoclonal
Clone M232
Isotype IgG1
Target Name eNOS
Antigen Species Mouse
Immunogen Synthetic peptide (coupled to carrier protein) around Ser632 of Mouse eNOS. This sequence is conserved in human (Ser-633) and rat (Ser-632) eNOS, and has low homology to other NOS family members.
Conjugation Un-conjugated
Full Name nitric oxide synthase 3, endothelial cell
Alternate Names Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:1000
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 133 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 1 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18127 Mouse NOS3

GeneID: 24600 Rat NOS3

GeneID: 4846 Human NOS3

Gene Symbol Nos3
Gene Full Name nitric oxide synthase 3, endothelial cell
Background Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation.
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
PTM Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

Images (1) Click the Picture to Zoom In

  • ARG20525 anti-eNOS phospho (Ser632) antibody [M232] WB image

    Western blot: 1) and 2) calyculin A (100 nM) treated Human umbilical vein endothelial cells for 30 min, 2) then the blots were treated with lambda phosphatase. The blots were stained with ARG20525 anti-eNOS phospho (Ser632) antibody [M232]. The antibody detects eNOS phospho (Ser632) at around 140 kda, and the 100 kda signal might be a non-specific signal.