ARG22244

anti-mGluR1 + mGluR5 antibody [S75-33]

anti-mGluR1 + mGluR5 antibody [S75-33] for Western blot,Immunohistochemistry,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [S75-33] recognizes mGluR1 + mGluR5
Tested Reactivity Hu, Ms, Rat
Tested Application IHC, IP, WB
Specificity Detects ~130kDa (both mGluR1 and mGluR5).
Host Mouse
Clonality Monoclonal
Clone S75-33
Isotype IgG1
Target Name mGluR1 + mGluR5
Antigen Species Rat
Immunogen Fusion protein around aa. 824-1203 (cytoplasmic C-terminus) of Rat mGluR5b
Conjugation Un-conjugated
Alternate Names PPP1R86; mGlu5; GPRC1E; Metabotropic glutamate receptor 5; mGluR5; MGLUR5

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:1000
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 108071 Mouse GRM5

GeneID: 24418 Rat GRM5

GeneID: 2915 Human GRM5

Gene Symbol Grm5
Gene Full Name glutamate receptor, metabotropic 5
Background This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Function G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity. [UniProt]
Cellular Localization Cell membrane
Calculated MW ~130 kDa (both mGluR1 and mGluR5).

Images (1) Click the Picture to Zoom In

  • ARG22244 anti-mGluR1 + mGluR5 antibody [S75-33] WB image

    Western blot: Rat brain membrane lysate stained with ARG22244 anti-mGluR1 + mGluR5 antibody [S75-33] at 1:1000 dilution.