ARG46748
anti-mScarlet antibody
anti-mScarlet antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoelectron Microscopy,Western blot and Other
Overview
| Product Description | Goat Polyclonal antibody recognizes mScarlet |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, IEM, IHC-Fr, IHC-P, WB |
| Specificity | This antibody does not recognize GFP (green fluorescent protein). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | mScarlet |
| Immunogen | Purified recombinant fluorescent protein produced in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Scarlet fluorescent protein; red fluorescent protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | Working dilution samples should be discarded if not used within 12 hours For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | This antibody specifically detects mScarlet in transfected cells and does not cross-react with GFP, making it highly suitable for multi-color fluorescence experiments. It is an excellent tool for applications such as protein tagging, viral transduction studies, and cellular imaging. mScarlet is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mScarlet is a ~26.5 kDa protein that is optimally excited at a 569 nm and has a maximum of emission at 594 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications. |
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| Highlight | Related news: tdTomato, mRuby, and mCherry antibodies for triple staining; |
| Calculated MW | 26.5 kDa |
Images (3) Click the Picture to Zoom In
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ARG46748 anti-mScarlet antibody IHC-Wmt image
Immunohistochemistry: Drosophila larvae NMJ muscle 6/7 expressing mScarlet in neurons (DyGlutmScarlet) using ARG46748 anti-mScarlet antibody at 1:1000. Secondary antibody: anti-goat conjugated to DyLight®488 at 1:500.
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ARG46748 anti-mScarlet antibody ICC/IF image
Immunocytochemistry: hCEC cells transfected with mScarlet-Rab5a and stained with ARG46748 anti-mScarlet antibody at 1:250 dilution. Cells were fixed with methanol.
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ARG46748 anti-mScarlet antibody WB image
Western blot: 50 μg of mScarlet plasmid transfected HEK293 cell lysate stained with ARG46748 anti-mScarlet antibody at 1:2500 dilution.
