ARG54677

anti-p19 INK4d antibody

anti-p19 INK4d antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes p19 INK4d
Tested Reactivity Hu, Ms
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name p19 INK4d
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide within 15-43 aa (N-terminus) of Human p19 INK4d protein (NP_001791.1).
Conjugation Un-conjugated
Alternate Names p19-INK4D; p19; Cyclin-dependent kinase 4 inhibitor D; p19-INK4d; INK4D

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse testis

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1032 Human CDKN2D

GeneID: 12581 Mouse CDKN2D

Swiss-port # P55273 Human Cyclin-dependent kinase 4 inhibitor D

Swiss-port # Q60773 Mouse Cyclin-dependent kinase 4 inhibitor D

Gene Symbol CDKN2D
Gene Full Name cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
Background P19 is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]
Cellular Localization Nucleus, Cytoplasm
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 18 kDa

Images (3) Click the Picture to Zoom In

  • ARG54677 anti-p19 INK4d antibody IHC image

    Immunohistochemistry: Formalin fixed and paraffin embedded testis tissue stained with ARG54677 anti-p19 INK4d antibody.

  • ARG54677 anti-p19 INK4d antibody WB image

    Western blot: 35 µg of Mouse testis lysate stained with ARG54677 anti-p19 INK4d antibody.

  • ARG54677 anti-p19 INK4d antibody FACS image

    Flow Cytometry: HeLa cells stained with ARG54677 anti-p19 INK4d antibody (right histogram) or without primary antibody control (left histogram).