ARG10519

anti-p53 antibody [Pab1801]

anti-p53 antibody [Pab1801] for ChIP,ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Radioimmunoassay,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
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anti-p53 antibody [Pab1801] Mouse Monoclonal antibody [Pab 1801] recognizes p53 ARG10519 Arigo biolaboratories
4.3 out of 5 based on 3
anti-p53 antibody [Pab1801] is rated 4.3 out of 5 by 3.

anti-p53 antibody [Pab1801]

Overview

Product Description Mouse Monoclonal antibody [Pab 1801] recognizes p53
Tested Reactivity Hu, Ms, Rat
Tested Application ChIP, ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, RIA, WB
Specificity The epitope of this antibody is located at aa 46-55 of the human p53 protein, so this antibody might react to both wild type and mutant form of p53 proteins.
Host Mouse
Clonality Monoclonal
Clone Pab 1801
Isotype IgG1
Target Name p53
Antigen Species Human
Immunogen Purified recombinant fragment within 32 to 79 aa of Human p53 protein (NP_000537.3).
Epitope Amino acids 46-55 of the human p53 protein
Conjugation Un-conjugated
Full Name tumor protein p53
Alternate Names TRP53; LFS1; Cellular tumor antigen p53; Phosphoprotein p53; Tumor suppressor p53; BCC7; Antigen NY-CO-13; P53

Application Instructions

Application Suggestion
Tested Application Dilution
ChIPAssay-dependent.
ELISAAssay-dependent.
FACS1µg per 10^6 cells.
ICC/IFAssay-dependent.
IHC-Fr4 - 10 µg/ml
IHC-P1 - 10 µg/ml
IP2 - 10 µg per mg of lysate.
RIAAssay-dependent.
WB0.5-5.0 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM citrate buffer (pH 6.0) for 10 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MDA-MB-231 cell line
Calculated MW 53 kDa

Properties

Form Liquid
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 24842 Rat TP53

GeneID: 7157 Human TP53

Swiss-port # P04637 Human Cellular tumor antigen p53

Swiss-port # P10361 Rat Cellular tumor antigen p53

Gene Symbol TP53
Gene Full Name tumor protein p53
Background P53 protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Highlight Related products:
p53 antibodies; p53 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Besides tumor suppression, what’s p53 busy for during embryogenesis?
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
PTM Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner.

Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-33 by CDK7 in a CAK complex in response to DNA damage. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP. Phosphorylated by NUAK1 at Ser-15 and Ser-392; was intially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1 (PubMed:21317932). It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated by DYRK2 at Ser-46 in response to genotoxic stress. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.

Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.

May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.

Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation (PubMed:10722742, PubMed:12810724, PubMed:15340061, PubMed:17170702, PubMed:19880522). Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:10722742, PubMed:12810724, PubMed:20173098). Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation (PubMed:19536131). Deubiquitinated by USP10, leading to its stabilization (PubMed:20096447). Ubiquitinated by TRIM24, RFFL, RNF34 and RNF125, which leads to proteasomal degradation (PubMed:19556538). Ubiquitination by TOPORS induces degradation (PubMed:19473992). Deubiquitination by USP7, leading to stabilization (PubMed:15053880). Isoform 4 is monoubiquitinated in an MDM2-independent manner (PubMed:15340061). Ubiquitinated by RFWD2, which leads to proteasomal degradation (PubMed:19837670). Ubiquitination and subsequent proteasomal degradation is negatively regulated by CCAR2 (PubMed:25732823).

Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by KMT5A, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Dimethylation at Lys-370 and Lys-382 diminishes p53 ubiquitination, through stabilizing association with the methyl reader PHF20. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation.

Sumoylated with SUMO1. Sumoylated at Lys-386 by UBC9. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG10519 anti-p53 antibody [Pab1801] WB image

    Western blot: 30 µg of 293T and MDAMB231 cell lysates stained with ARG10519 anti-p53 antibody [Pab1801] at 1:500 dilution.

  • ARG10519 anti-p53 antibody [Pab1801] WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG10519 anti-p53 antibody [Pab1801] at 1:500 dilution.

  • ARG10519 anti-p53 antibody [Pab1801] WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG10519 anti-p53 antibody [Pab1801] at 2 µg/ml dilution.

Customer's Feedback

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Review for anti-p53 antibody [Pab1801]

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:2 μg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Excellent

Review for anti-p53 antibody [Pab1801]

Application:WB

Sample:A: DU145, B: PC3 (p53 null cell line)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Good

Review for anti-p53 antibody [Pab1801]

Application:WB

Sample:293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

Clone References

The role of the HIF-1α transcription factor in increased cell division at physiological oxygen tensions.

WB / Human

Carrera S et al.
PLoS One.,  (2014)

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p53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (ER) stress.

WB / Human

Thomas SE et al.
J Biol Chem.,  (2013)

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Reactive oxygen species and mitochondrial sensitivity to oxidative stress determine induction of cancer cell death by p21.

WB / Human

Masgras I et al.
J Biol Chem.,  (2012)

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Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study.

IHC / Human

Fan C et al.
Tumour Biol.,  (2012)

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