ARG10582

anti-p60 antibody [E17-L]

anti-p60 antibody [E17-L] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Monoclonal antibody [E17-L] recognizes p60
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone E17-L
Isotype IgG
Target Name p60
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human p60
Conjugation Un-conjugated
Full Name chromatin assembly factor 1, subunit B (p60)
Alternate Names CAF1A; CAF-IP60; CAF-I 60 kDa subunit; MPP7; MPHOSPH7; CAF1; CAF-1; Chromatin assembly factor I p60 subunit; M-phase phosphoprotein 7; CAF1P60; Chromatin assembly factor 1 subunit B; CAF-1 subunit B; CAF-I p60

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 20 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 8208 Human CHAF1B

Swiss-port # Q13112 Human Chromatin assembly factor 1 subunit B

Gene Symbol CHAF1B
Gene Full Name chromatin assembly factor 1, subunit B (p60)
Background Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
Function Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. [UniProt]
PTM Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.

Images (2) Click the Picture to Zoom In

  • ARG10582 anti-p60 antibody [E17-L] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human lymphocytes of the germinal center of the lymph node (4 µm sections) stained with ARG10582 anti-p60 antibody [E17-L].

  • ARG10582 anti-p60 antibody [E17-L] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human colorectal adenocarcinoma (4 µm sections) stained with ARG10582 anti-p60 antibody [E17-L].