ARG10654

anti-p63 antibody

anti-p63 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes p63

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name p63
Antigen Species Human
Immunogen Synthetic peptide around aa. 82-98 of Human p63. (DMDARRNKQQRIKEEGE)
Conjugation Un-conjugated
Full Name tumor protein p63
Alternate Names p63; Tumor protein p73-like; B(p51A); AIS; p53CP; p73L; p73H; p40; EEC3; TP63; NBP; Chronic ulcerative stomatitis protein; TP53CP; CUSP; B(p51B); TP73L; p51; Transformation-related protein 63; Keratinocyte transcription factor KET; SHFM4; TP53L; RHS; LMS; Tumor protein 63; OFC8; KET

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF0.5 - 1 µg/ml
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note The antibody is not suitable for mouse and rat samples by IHC-P application (might get week signal).

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 246334 Rat TP63

GeneID: 8626 Human TP63

Swiss-port # Q9H3D4 Human Tumor protein 63

Swiss-port # Q9JJP6 Rat Tumor protein 63

Gene Symbol TP63
Gene Full Name tumor protein p63
Background This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq, Jul 2008]
Function Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. [UniProt]
PTM May be sumoylated.
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Images (6) Click the Picture to Zoom In

  • ARG10654 anti-p63 antibody ICC/IF image

    Immunocytochemistry: A549 cells stained with ARG10654 anti-p63 antibody.

  • ARG10654 anti-p63 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human esophageal squamous cell carcinoma tissue stained with ARG10654 anti-p63 antibody.

  • ARG10654 anti-p63 antibody WB image

    Western blot: 1) HeLa, 2) SMMC-7721, 3) COLO320, 4) A549, and 5) SGC-7901 cell lysates stained with ARG10654 anti-p63 antibody.

  • ARG10654 anti-p63 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human esophageal squamous cell carcinoma tissue stained with ARG10654 anti-p63 antibody.

  • ARG10654 anti-p63 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG10654 anti-p63 antibody.

  • ARG10654 anti-p63 antibody WB image

    Western blot: 50 µg of 1) Rat heart and 2) Mouse thymus lysates stained with ARG10654 anti-p63 antibody at 0.5 µg/ml dilution.