ARG51740

anti-p70 S6 Kinase phospho (Thr421) antibody

anti-p70 S6 Kinase phospho (Thr421) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes p70 S6 Kinase phospho (Thr421)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name p70 S6 Kinase
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of threonine 421 (P-R-T(p)-P-V) derived from Human p70 S6 Kinase.
Conjugation Un-conjugated
Alternate Names p70 S6 kinase alpha; p70(S6K)-alpha; p70-alpha; p70 S6KA; S6K; STK14A; S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; S6K-beta-1; P70S6K1; PS6K; p70 ribosomal S6 kinase alpha; p70-S6K; EC 2.7.11.1; Serine/threonine-protein kinase 14A; p70 S6K-alpha; Ribosomal protein S6 kinase beta-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6198 Human RPS6KB1

GeneID: 72508 Mouse RPS6KB1

GeneID: 83840 Rat RPS6KB1

Gene Symbol RPS6KB1
Gene Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Background Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
Function Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 59 kDa
PTM Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.

Images (3) Click the Picture to Zoom In

  • ARG51740 anti-p70 S6 Kinase phospho (Thr421) antibody WB image

    Western blot: Extracts from Jurkat cells untreated(lane 1) or treated with GM-CSF(lane 2) stained with ARG51740 anti-p70 S6 Kinase phospho (Thr421) antibody.

  • ARG51740 anti-p70 S6 Kinase phospho (Thr421) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51740 anti-p70 S6 Kinase phospho (Thr421) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51740 anti-p70 S6 Kinase phospho (Thr421) antibody ICC/IF image

    Immunofluorescence: methanol-fixed MCF cells stained with ARG51740 anti-p70 S6 Kinase phospho (Thr421) antibody.