ARG22357

anti-pan FGFA / FHFA antibody [S235-22]

anti-pan FGFA / FHFA antibody [S235-22] for Western blot,ICC/IF and Human,Rat

Overview

Product Description Mouse Monoclonal antibody [S235-22] recognizes pan FGFA / FHFA
Tested Reactivity Hu, Rat
Predict Reactivity Ms
Tested Application ICC/IF, WB
Specificity Does not cross-react with FGF13B / FHF2B. Cross reacts with FGF12A / FHF1A and FGF14A / FHF4A.
Host Mouse
Clonality Monoclonal
Clone S235-22
Isotype IgG2b
Target Name pan FGFA / FHFA
Antigen Species Human
Immunogen Synthetic peptide around aa. 2-18 of Human FHF2A (AAAIASSLIRQKRQARE). 100% identical to Rat, 94% identical to Mouse. > 80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A.
Conjugation Un-conjugated
Alternate Names FHF-2; FHF2; Fibroblast growth factor 13; Fibroblast growth factor homologous factor 2; FGF-13; FGF2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol.
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2258 Human FGF13

GeneID: 84488 Rat FGF13

Swiss-port # Q92913 Human Fibroblast growth factor 13

Swiss-port # Q9ERW3 Rat Fibroblast growth factor 13

Gene Symbol FGF13
Gene Full Name fibroblast growth factor 13
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini. [provided by RefSeq, Nov 2008]
Function Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus.

May regulate voltage-gated sodium channels transport and function.

May also play a role in MAPK signaling. [UniProt]
Calculated MW 28 kDa
PTM May be phosphorylated.

Images (2) Click the Picture to Zoom In

  • ARG22357 anti-pan FGFA / FHFA antibody [S235-22] ICC/IF image

    Immunocytochemistry: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Cells were stained with ARG22357 anti-pan FGFA / FHFA antibody [S235-22] at 1:100 dilution (60 min, RT). Magnification: 60X. (A) DAPI (blue) nuclear stain, (B) Phalloidin Texas Red F-Actin stain, (C) ARG22357 anti-pan FGFA / FHFA antibody [S235-22] (green), and (D) Composite.

  • ARG22357 anti-pan FGFA / FHFA antibody [S235-22] WB image

    Western blot: 15 µg of Rat brain membrane stained with ARG22357 anti-pan FGFA / FHFA antibody [S235-22] at 1:200 dilution (16 hours, 4°C). Block: 2% BSA and 2% Skim Milk in 1X TBST. .