anti-tdTomato antibody

anti-tdTomato antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Other

publication_link Publication3


Product Description

Goat Polyclonal antibody recognizes tdTomato

Tested Reactivity Other
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody recognizes tdTomato, mCherry, mApple and red fluorescent protein (DsRed). It does not cross-react to GFP.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name tdTomato
Immunogen Purified full-length recombinant protein produced in E. coli.
Conjugation Un-conjugated
Alternate Names Cherry fluorescent protein; DsRed; mCherry; red fluorescent protein; RFP

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Background tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It's brightness and emission wavelength makes it ideal for live animal research.

Images (4) Click the Picture to Zoom In

  • ARG55724 anti-tdTomato antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG55724 anti-tdTomato antibody at 1:100 dilution.

    From Jonathan Ribeiro et al. Nucleic Acids Res. (2023), doi: 10.1093/nar/gkad168, Fig. S5.

  • ARG55724 anti-tdTomato antibody ICC/IF image

    Immunofluorescence: N2a cells transfected with tdTomoto reporter plasmid and stained with ARG55724 anti-tdTomato antibody at 1:200 dilution. Cells were fixed with 4% of PFA.
    Cell nuclei were stained with DAPI (blue).

  • ARG55724 anti-tdTomato antibody IHC-P image

    Immunohistochemistry:Paraffin-embedded Mouse Oviducts stained with ARG55724 anti-tdTomato antibody at 1:500 dilution (overnight at 4°C). Secondary antibody: Alexa Fluor 488-Goat anti-Goat (green) at 1:500 dilution (2 hours at RT).

  • ARG55724 anti-tdTomato antibody WB image

    Western blot: 50μg of mApple plasmid transfected HEK293 cell lysate stained with ARG55724 anti-tdTomato antibody at 1:2500 dilution.

Specific References

Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

IHC-Fr / Mouse

Qijun Tang et al.
Sci Adv.,  (2023)




GCNA is a histone binding protein required for spermatogonial stem cell maintenance

IHC-P / Mouse

Jonathan Ribeiro et al.
Nucleic Acids Res.,  (2023)




BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury

ICC/IF, IHC-free-floating / Mouse

Mengke Fan et al.
Cell Mol Gastroenterol Hepatol,  (2022)