ARG70605
arigoPrime+ ECL HRP Substrate
arigoPrime+ ECL HRP Substrate for Western blot
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG70605-001 | arigoPrime+ ECL HRP Substrate Solution A | 50 / 250 ml (Ready to use) | 4°C |
| ARG70605-002 | arigoPrime+ ECL HRP Substrate Solution B | 50 / 250 ml (Ready to use) | 4°C (Protect from light) |
Overview
| Product Description | arigoPrime+ ECL HRP Substrate is a highly sensitive, non-radioactive, enhanced luminol-based chemiluminescent substrate (ECL) detection reagent. It is particularly useful for detecting horseradish peroxidase (HRP)-conjugated antibodies on immunoblotting and other immunoassay applications. arigoPrime+ ECL HRP Substrateis based on the oxidation of luminol catalyzed by HRP, producing a chemiluminescent signal that can be visualized using X-ray film or chemiluminescence imaging systems (e.g., Digital charge-coupled device (CCD) camera imaging devices) |
|---|---|
| Tested Application | WB |
| Sensitivity | Picogram |
Properties
| Form | Liquid |
|---|---|
| Storage Instruction | Store at 4-8ºC and protect from light. Do not freeze. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (2) Click the Picture to Zoom In
-
Western blot analysis using arigoPrime+ ECL HRP Substrate
The indicated amounts of human cell lysates were separated by 12% Bis-Tris SDS-PAGE and transferred to a PVDF membrane. After blocking, the membrane was probed with primary antibody rabbit anti-beta Actin Cat. No. ARG65683 diluted 1:5000 and secondary antibody goat anti-rabbit IgG (HRP) Cat. No. ARG65351 diluted 1:10000.
-
Western blot analysis using arigoPrime+ ECL HRP Substrate
The indicated amounts of recombinant human IL1 beta protein was separated by 12% Bis-Tris SDS-PAGE and transferred to a PVDF membrane. After blocking, the membrane was probed with primary antibody mouse anti-IL1 beta Cat. No. ARG66285 diluted 1:3000 and secondary antibody goat anti-mouse IgG (HRP) Cat. No. ARG65350 diluted 1:5000.
Customer's Feedback
Excellent
Review for arigoPrime+ ECL HRP Substrate
Application：WB
Sample：Recombinant human IL1 beta protein
Sample Loading Amount：39.06 - 20000 pg
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for arigoPrime+ ECL HRP Substrate
Application：WB
Sample：Human cell lysates
Sample Loading Amount：19.53 - 10000 ng
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
| Title | Download Link |
|---|---|
| ARG70605 arigoPrime+ ECL HRP Substrate User-manual |
 Download
|
