ARG83771

arigoQIK® Human CD47 ELISA Development Kit

arigoQIK® Human CD47 ELISA Development Kit for ELISA and Human

Overview

Product Description ARG83771 arigoQIK® Human CD47 ELISA Development Kit, includes Capture antibody, Detection antibody, Standard, and HRP-Streptavidin Solution.
This ELISA Development Kit is designed for the development of sandwich ELISA to measure Human CD47 in Serum, plasma and cell culture supernatants.

For other reagents required for arigoQIK® ELISA Development Kit, please refer ARG83524 Integral Reagent Kit (ELISA Development Kit)

More aboutarigoQIK® :
● Optimized capture and detection antibody pairs
● Reduced incubation time and wash cycles
● 2-hour quicker than conventional ELISA process
● 5- and 15-plate packages available
Tested Reactivity Hu
Tested Application ELISA
Target Name CD47
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 62.50 - 4000 pg/ml
Sample Volume 50 μL
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 10%
Alternate Names Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein

Properties

Storage Instruction Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 961 Human CD47

Swiss-port # Q08722 Human Leukocyte surface antigen CD47

Gene Symbol CD47
Gene Full Name CD47 molecule
Background This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
Function Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt]
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