ARG83717
arigoQIK® Human OPN / Osteopontin ELISA Development Kit
arigoQIK® Human OPN / Osteopontin ELISA Development Kit for ELISA and Human
Overview
| Product Description | ARG83717 arigoQIK® Human OPN / Osteopontin ELISA Development Kit, includes Capture antibody, Detection antibody, Standard, and HRP-Streptavidin Solution. This ELISA Development Kit is designed for the development of sandwich ELISA to measure Human OPN / Osteopontin in Serum, plasma and cell culture supernatants. For other reagents required for arigoQIK® ELISA Development Kit, please refer ARG83524 Integral Reagent Kit (ELISA Development Kit) More aboutarigoQIK® : ● Optimized capture and detection antibody pairs ● Reduced incubation time and wash cycles ● 2-hour quicker than conventional ELISA process ● 5- and 15-plate packages available |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | OPN / Osteopontin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.16 pg/mL |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 0.3125-20 pg/mL |
| Sample Volume | 50 μL |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | SPP1; Secreted Phosphoprotein 1; Lnc-PKD2-2-3; Osteopontin; ETA-1; BSPI; BNSP; OPN; Early T-Lymphocyte Activation 1; Urinary Stone Protein; Nephropontin; Uropontin; Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early T-Lymphocyte Activation 1); Osteopontin/Immunoglobulin Alpha 1 Heavy Chain Constant Region Fusion Protein; Bone Sialoprotein I; SPP1/CALPHA1 Fusion; Bone Sialoprotein 1; SPP-1 |
Properties
| Storage Instruction | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
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| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | SPP1 |
| Gene Full Name | Secreted Phosphoprotein 1 |
| Background | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Function | Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [Uniprot] |
| Title | Download Link |
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| ARG83717 arigoQIK® Human OPN / Osteopontin ELISA Development Kit User manual |
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