ARG83729
arigoQIK® Mouse CD274 / PD-L1 ELISA Development Kit
arigoQIK® Mouse CD274 / PD-L1 ELISA Development Kit for ELISA and Mouse
Overview
| Product Description | ARG83729 arigoQIK® Mouse CD274 / PD-L1 ELISA Development Kit, includes Capture antibody, Detection antibody, Standard, and HRP-Streptavidin Solution. This ELISA Development Kit is designed for the development of sandwich ELISA to measure Mouse CD274 / PD-L1 in Serum, plasma and cell culture supernatants. For other reagents required for arigoQIK® ELISA Development Kit, please refer ARG83524 Integral Reagent Kit (ELISA Development Kit) More aboutarigoQIK® : ● Optimized capture and detection antibody pairs ● Reduced incubation time and wash cycles ● 2-hour quicker than conventional ELISA process ● 5- and 15-plate packages available |
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| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | CD274 / PD-L1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 4 pg/mL |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 7.81-500 pg/mL |
| Sample Volume | 50 μL |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | CD274; CD274 Molecule; PD-L1; B7H1; PDL1; Programmed Cell Death 1 Ligand 1; CD274 Antigen; B7 Homolog 1; PDCD1LG1; B7-H1; B7-H; PDCD1 Ligand 1; PDCD1L1; HPD-L1; Programmed Death Ligand 1 |
Properties
| Storage Instruction | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
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| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CD274 |
| Gene Full Name | CD274 Molecule |
| Background | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [Uniprot] |
| Title | Download Link |
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| ARG83729 arigoQIK® Mouse CD274 / PD-L1 ELISA Development Kit User manual |
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