ARG83728
arigoQIK® Mouse Leptin ELISA Development Kit
arigoQIK® Mouse Leptin ELISA Development Kit for ELISA and Mouse
Overview
| Product Description | ARG83728 arigoQIK® Mouse Leptin ELISA Development Kit, includes Capture antibody, Detection antibody, Standard, and HRP-Streptavidin Solution. This ELISA Development Kit is designed for the development of sandwich ELISA to measure Mouse Leptin in Serum, plasma and cell culture supernatants. For other reagents required for arigoQIK® ELISA Development Kit, please refer ARG83524 Integral Reagent Kit (ELISA Development Kit) More aboutarigoQIK® : ● Optimized capture and detection antibody pairs ● Reduced incubation time and wash cycles ● 2-hour quicker than conventional ELISA process ● 5- and 15-plate packages available |
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| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | Leptin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.4 pg/mL |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 0.625-40 pg/mL |
| Sample Volume | 50 μL |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | LEP; Leptin; OBS; OB; Leptin (Murine Obesity Homolog); Leptin (Obesity Homolog, Mouse); Obesity Factor; Obese Protein; Obese, Mouse, Homolog Of; LEPD |
Properties
| Storage Instruction | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
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| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | LEP |
| Gene Full Name | Leptin |
| Background | This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017] |
| Function | Increases CD4+CD25- T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation. [Uniprot] |
| Title | Download Link |
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| ARG83728 arigoQIK® Mouse Leptin ELISA Development Kit User manual |
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