ARG83732

arigoQIK® Rat IL6 ELISA Development Kit

arigoQIK® Rat IL6 ELISA Development Kit for ELISA and Rat

Overview

Product Description ARG83732 arigoQIK® Rat IL6 ELISA Development Kit, includes Capture antibody, Detection antibody, Standard, and HRP-Streptavidin Solution.
This ELISA Development Kit is designed for the development of sandwich ELISA to measure Rat IL6 in Serum, plasma and cell culture supernatants.

For other reagents required for arigoQIK® ELISA Development Kit, please refer ARG83524 Integral Reagent Kit (ELISA Development Kit)

More aboutarigoQIK® :
● Optimized capture and detection antibody pairs
● Reduced incubation time and wash cycles
● 2-hour quicker than conventional ELISA process
● 5- and 15-plate packages available
Tested Reactivity Rat
Tested Application ELISA
Target Name IL6
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 35 pg/mL
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 62.5-4000 pg/mL
Sample Volume 50 μL
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 10%
Alternate Names IL6; Interleukin 6; IL-6; IFNB2; BSF2; HGF; HSF; B-Cell Stimulatory Factor 2; CTL Differentiation Factor; Hybridoma Growth Factor; Interferon Beta-2; Interleukin-6; IFN-Beta-2; BSF-2; CDF; Interleukin 6 (Interferon, Beta 2); B-Cell Differentiation Factor; Interferon, Beta 2; Interleukin BSF-2

Properties

Storage Instruction Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24498 Rat IL6

Swiss-port # P20607 Rat Interleukin-6

Gene Symbol IL6
Gene Full Name Interleukin 6
Background This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]
Function Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration. [Uniprot]
Title Download Link
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