HMGB1

HMGB1

 

HMGB1 is a multifunctional protein with various roles in different cellular compartments. In the nucleus, HMGB1 acts as a DNA-binding protein for gene transcription, gene regulation and DNA repair. In the cytosol, HMGB1 induces autophagy by interacting with Beclin-1 which results in its dissociation from Bcl2 and the subsequent induction of autophagy. In the extracellular compartment, HMGB1 is passively released by necrotic cells and acts as a chemokine and DAMP (Damage-associated molecular pattern) to trigger inflammatory responses. Besides, HMGB1 is also secreted from activated dentric cells and macrophages and functions as a cytokine, it acts as a ligand for RAGE, TLR-2 and TLR-4 thus activating the signal pathways and the following inflammation responses. The HMGB1 released from apoptotic cells is neutralized by reactive oxygen species (ROS)-dependent oxidation and thus promoting immunological tolerance. HMGB1 has attracted the attention of both basic and clinical researchers as its association with various pathologies such as cancer, sepsis, arteriosclerosis, chronic rheumatoid arthritis and nephritis.

arigo offers the best ELISA kits and antibodies to facilitate the HMGB1-related research.

 

The most comprehensive HMGB1 ELISA kits

* HMGB1 specific. No cross reactivity with HMGB2.
* Suitable for multiple samples from multiple species.
* Easy & Fast by steps of 2 incubations and 1 wash cycle.

 

Human HMGB1 ELISA Kit (ARG81185)
Samples:
Plasma and cell culture supernatant.
Citations:
Riegger et al., (2019) Cell Death Dis. 10(10):683.
Di et al., (2019) J Gastroenterol Hepatol. 34(4):728-735.

 


Mouse/Rat HMGB1 ELISA Kit (ARG81310)
Samples:
Plasma, cell culture supernatant and cell/tissue lysate.
Citations:
Song et al., (2019) Nat Commun. 10(1):3745.
Ji et al., (2019) Aging. 11(13):4338-4353.
Takahashi et al., (2019) Oncotarget. 10(6):633-646.
He et al., (2019) J Cell Physiol. 234(10):17314-17325.

 

HMGB1 ELISA Kit (ARG81351)
Samples:
Serum from human, mouse, or rat.
Citations:
Akasaka et al., (2019) Int J Mol Sci. 20(21). pii: E5462.

 

The best HMGB1 antibodies

HMGB1 antibody [SQab1711] (ARG65863)
Mouse mAb
Reactivity:
Hu, Ms, Rat
Application: ELISA, FACS, ICC/IF, IHC-P, WB

HMGB1 antibody (ARG65636)
Rabbit pAb
Reactivity:
Hu, Ms, Rat
Application: IHC-P, WB