ARG30269

Apoptosis Marker Antibody Duo (Bcl2, Bid)

publication_link Publication9

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG20055 anti-Bid antibody Rabbit pAb Hu IHC, IP, WB 25 μg
ARG55188 anti-Bcl 2 antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μl

Overview

Product Description

Bcl2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.

Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid protein is a member of the BCL2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. (provided by RefSeq, Jul 2008)

Bcl2, BID and Bax are known to play a major role in the process of apoptosis and their dysfunction underlies carcinogenesis. Therefore, anti-Bcl2, Bid and Bax antibodies have been used in many cancer and cancer therapeutic studies.

arigo provide an Apoptosis marker Duo, ARG30269, including anti-Bcl2 and anti-Bid antibodies, is useful for user to study the correlation of Bid, Bcl2 and apoptosis.

Target Name Apoptosis Marker
Alternate Names Apoptosis Marker antibody; Bid antibody; Bcl 2 antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (6) Click the Picture to Zoom In

  • ARG55188 anti-Bcl 2 antibody WB image

    Western blot: 30 μg of Jurkat cell lysate stained with ARG55188 anti-Bcl 2 antibody at 1:1000 dilution.

  • ARG20055 anti-Bid antibody WB image

    Western blot: 30 µg of Jurkat cell lysate stained with ARG20055 anti-Bid antibody at 1:500 dilution.

  • ARG55188 anti-Bcl 2 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55188 anti-Bcl 2 antibody (red) at 1:100 dilution. DAPI (blue) for nuclear staining.

  • ARG55188 anti-Bcl 2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55188 anti-Bcl 2 antibody at 1:200 dilution.

  • ARG55188 anti-Bcl-2 antibody IHC image

    Immunohistochemistry: paraffin-embedded rat brain stained with ARG55188 anti-Bcl-2 antibody at 1:200 dilution.

  • ARG55188 anti-Bcl-2 antibody WB image

    Western blot: 30 μg of Jurkat cell lysate stained with ARG55188 anti-Bcl-2 antibody at 1:1000 dilution.

Specific References

Naringin Interferes Doxorubicin-Induced Myocardial Injury by Promoting the Expression of ECHS1

ARG55188: WB / Rat

Zirui Zhao et al.
Front Pharmacol.,  (2022)

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Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers

ARG55188: WB / Human

Tzu-Chun Kan et al.
Pharmaceutics.,  (2022)

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Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

ARG55188: WB / Human

Chen-Sheng Chen et al.
Int J Mol Sci.,  (2021)

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Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG55188: WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

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Dapagliflozin Improves Cardiac Hemodynamics and Mitigates Arrhythmogenesis in Mitral Regurgitation-Induced Myocardial Dysfunction.

ARG55188: WB / Rat

Yu-Wen Lin et al.
J Am Heart Assoc.,  (2021)

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Xenogeneic and Allogeneic Mesenchymal Stem Cells Effectively Protect the Lung Against Ischemia-reperfusion Injury Through Downregulating the Inflammatory, Oxidative Stress, and Autophagic Signaling Pathways in Rat.

ARG55188: WB / Human

Kun-Chen Lin et al.
Cell Transplant.,  (2020)

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Possible Involvement of PTEN Signaling Pathway in the Anti-apoptotic Effect of Electroacupuncture Following Ischemic Stroke in Rats

ARG55188: WB / Rat

Xing Ying et al.
Cell Mol Neurobiol.,  (2018)

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Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.

ARG55188: WB / Rat

Xing Ying et al.
J Mol Neurosci.,  (2018)

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A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis.

ARG55188: WB / Mouse

Wang Xuan et al.
Oncology Letters.,  (2018)

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