ARG21905

Goat anti-Human IgG antibody (Biotin), pre-adsorbed

Goat anti-Human IgG antibody (Biotin), pre-adsorbed for Depletion,ELISA,ELISPOT,FLISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Human IgG
Tested Reactivity Hu
Tested Application Depletion, ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The antibody reacts with the heavy chain of Human IgG. The antibody is pre-adsorbed with Human IgM and IgA, so the antibody may not react with Human IgM and IgA, but may react with IgG from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG
Antigen Species Human
Conjugation Biotin

Application Instructions

Pre Adsorbed Human IgM and IgA.
Application Suggestion
Tested Application Dilution
DepletionAssay-dependent
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (1) Click the Picture to Zoom In

  • ARG21905 Goat anti-Human IgG antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG21905 Goat anti-Human IgG antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).