ARG21650

Goat anti-Human IgM antibody (Biotin), pre-adsorbed

Goat anti-Human IgM antibody (Biotin), pre-adsorbed for Depletion,ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Purification,Western blot and Human

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Human IgM
Tested Reactivity Hu
Tested Application Depletion, ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, Puri, WB
Specificity The antibody reacts with the heavy chain of Human IgM. The antibody is pre-adsorbed with Human IgG and IgA, so the antibody may not react with Human IgG and IgA, but may react with IgM from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Human IgM
Antigen Species Human
Conjugation Biotin

Application Instructions

Pre Adsorbed Human IgG and IgA.
Application Suggestion
Tested Application Dilution
DepletionAssay-dependent
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
PuriAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21650 Goat anti-Human IgM antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ARG21650 Goat anti-Human IgM antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG21650 Goat anti-Human IgM antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG21650 Goat anti-Human IgM antibody (Biotin) (pre-adsorbed) and ARG21260 anti-CD19 antibody [SJ25-C1] (PE) followed by Streptavidin (FITC).