ARG21553

Goat anti-Mouse Kappa Light Chain antibody (Biotin), pre-adsorbed

Goat anti-Mouse Kappa Light Chain antibody (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Mouse Kappa Light Chain
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The antibody reacts with Mouse kappa light chains. The antibody is pre-adsorbed with Mouse lambda light chains, so the antibody may not react with Mouse lambda light chains, but may react with kappa light chains from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Kappa Light Chain
Antigen Species Mouse
Conjugation Biotin

Application Instructions

Pre Adsorbed Mouse lambda light chains.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (4) Click the Picture to Zoom In

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Cytokeratin 18 antibody (right), followed by ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) followed by Streptavidin (FITC).

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Cytokeratin 18 antibody (right), followed by ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.