ARG80117

Human IFN gamma ELISA Kit

Human IFN gamma ELISA Kit for ELISA and Human

Cancer kit; Developmental Biology kit; Immune System kit; Signaling Transduction kit
publication_link Publications2

Component

Cat No Component Name Package Temp
ARG80117-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80117-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80117-003 Standard diluent buffer 20 ml 4°C
ARG80117-004 Antibody conjugate concentrate 400 µl 4°C
ARG80117-005 Antibody diluent buffer 16 ml 4°C
ARG80117-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80117-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80117-008 20X Wash buffer 50 ml 4°C
ARG80117-009 TMB substrate 12ml 4°C (Protect from light)
ARG80117-010 STOP solution 12ml 4°C
ARG80117-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80117 Human IFN gamma ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IFN gamma in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Canine IFN- gamma;cotton rat IFN- gamma;equine IFN- gamma;feline IFN- gamma;mouse IFN- gamma;porcine IFN- gamma;rat IFN- gamma
Target Name IFN gamma
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name interferon, gamma
Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IFNG
Gene Full Name interferon, gamma
Background This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
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Research Area Cancer kit; Developmental Biology kit; Immune System kit; Signaling Transduction kit
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

Images (1) Click the Picture to Zoom In

  • ARG80117 Human IFN gamma ELISA Kit standard curve image

    ARG80117 Human IFN gamma ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Clinical Significance of Decreased Interleukin-35 Expression in Patients with Psoriasis.

ELISA / Human

Li Teng-Da et al.
Microbiol Immunol.,  (2018)

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Anti-VEGFR2-Interferon-α2 regulates the tumor microenvironment and exhibits potent anti-tumor efficacy against colorectal cancer

ELISA / Human

Zhaoting Li et al.
OncoImmunology,  (2017)

publication_link

 

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Title Download Link
ARG80117 Human IFN gamma ELISA Kit User's manual download icon Download