ARG82010

Human MMP7 (active) ELISA Kit

Human MMP7 (active) ELISA Kit for ELISA and Human

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Component

Cat No Component Name Package Temp
ARG82010-001 Antibody-coated microplate 12 X 8 strips 4°C
ARG82010-002 Standard (200 ng) 1 vial 4°C, store at -20°C after reconstitution
ARG82010-003 Standard reconstitution buffer 1.1 ml 4°C
ARG82010-004 HRP-antibody Conjugate (200X) 65 µl 4°C
ARG82010-005 Sample diluent 15 ml 4°C
ARG82010-006 Assay buffer 12 ml 4°C
ARG82010-007 Antibody diluent 12 ml 4°C
ARG82010-008 10X Wash buffer 30 ml 4°C
ARG82010-009 TMB substrate 12 ml (ready-to-use) 4°C (Protect from light)
ARG82010-010 Stop solution 6 ml 4°C
ARG82010-011 Plate sealer 1 strip Room temperature

Overview

Product Description ARG82010 Human MMP7 (active) ELISA Kit is an Enzyme Immunoassay kit for the quantification of human MMP7 active form protein in serum, plasma, saliva, urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No specific signal was detected when using pro-MMP7 recombinant protein (20 ng/ml)
Target Name MMP7 (active)
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.102 ng/ml
Sample Type Serum, plasma, saliva, urine and cell culture supernatants.
Standard Range 0.3125 - 20 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 8.9%; Inter-Assay CV: 7.6%
Alternate Names MMP-7; Uterine metalloproteinase; Matrix metalloproteinase-7; EC 3.4.24.23; Matrilysin; Matrin; Pump-1 protease; PUMP-1; MPSL1

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4316 Human MMP7

Swiss-port # P09237 Human Matrilysin

Gene Symbol MMP7
Gene Full Name matrix metallopeptidase 7
Background MMP7 is a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. [provided by RefSeq, Jan 2016]
Function MMP7 degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Highlight Related products:
MMP7 antibodies; MMP7 ELISA Kits;
Related news:
New MMP7 (total or active) ELISA kits are released
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (2) Click the Picture to Zoom In

  • ARG82010 Human MMP7 (active) ELISA Kit standard curve image

    ARG82010 Human MMP7 (active) ELISA Kit results of a typical standard run with optical density reading at 450 nm.

  • ARG82010 Human MMP7 (active) ELISA Kit standard curve image

    ARG82010 Human MMP7 (active) ELISA Kit results of a typical standard run with optical density reading at 450 nm. Red: Active MMP7; Green: Pro-MMP7.

Specific References

Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis

ELISA / Human / Cell culture supernatants

Shen-Hsing Hsu et al.
Int J Mol Sci.,  (2022)

publication_link

 

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