ARG80120

Human TNF alpha ELISA Kit

Human TNF alpha ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit

Component

Cat No Component Name Package Temp
ARG80120-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80120-002 Standard (Lyophilized) 3 X 0.5 ng/vial 4°C
ARG80120-003 Standard diluent buffer 20 ml 4°C
ARG80120-004 Antibody conjugate concentrate 400 µl 4°C
ARG80120-005 Antibody diluent buffer 16 ml 4°C
ARG80120-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80120-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80120-008 20X Wash buffer 50 ml 4°C
ARG80120-009 TMB substrate 12ml 4°C (Protect from light)
ARG80120-010 STOP solution 12ml 4°C
ARG80120-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80120 Human TNF alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TNF alpha in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity This ELISA kit suitable for soluble human TNF-alpha detection, and no significant cross-reactivity or interference with Human TNF- beta, TNF RI, TNF RII;mouse TNF- alpha, TNF RI, TNF RII;porcine TNF- alpha;rat TNF- alpha
Target Name TNF alpha
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 7.8 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name tumor necrosis factor
Alternate Names Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol TNF
Gene Full Name tumor necrosis factor
Background This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Images (1) Click the Picture to Zoom In

  • ARG80120 Human TNF alpha ELISA Kit standard curve image

    ARG80120 Human TNF alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80120 Human TNF alpha ELISA Kit User's manual download icon Download