Mouse CD274 / PD-L1 ELISA Kit

Mouse CD274 / PD-L1 ELISA Kit for ELISA and Mouse

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Cat No Component Name Package Temp
ARG81930-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81930-002 Standard 2 X 10 ng/vial 4°C
ARG81930-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81930-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81930-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81930-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81930-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81930-008 25X Wash buffer 20 ml 4°C
ARG81930-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81930-010 STOP solution 10 ml (Ready to use) 4°C
ARG81930-011 Plate sealer 4 strips Room temperature


Product Description ARG81930 Mouse CD274 / PD-L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD274 / PD-L1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD274 / PD-L1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%; Inter-Assay CV: 7.5%
Alternate Names Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1

Application Instructions

Assay Time ~ 5 hours


Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 60533 Mouse CD274

Swiss-port # Q9EP73 Mouse Programmed cell death 1 ligand 1

Gene Symbol CD274
Gene Full Name CD274 molecule
Background CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG81930 Mouse CD274 / PD-L1 ELISA Kit standard curve image

    ARG81930 Mouse CD274 / PD-L1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

WEE1 inhibitor and ataxia telangiectasia and RAD3-related inhibitor trigger stimulator of interferon gene-dependent immune response and enhance tumor treatment efficacy through programmed death-ligand 1 blockade

ELISA / Mouse / cell culture supernatant

Xue Wu et al.
Cancer Sci.,  (2021)



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