ARG81930

Mouse CD274 / PD-L1 ELISA Kit

Mouse CD274 / PD-L1 ELISA Kit for ELISA and Mouse

Overview

Product Description ARG81930 Mouse CD274 / PD-L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD274 / PD-L1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD274 / PD-L1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 pg/ml - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%; Inter-Assay CV: 7.5%
Full Name CD274 molecule
Alternate Names Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 60533 Mouse CD274

Swiss-port # Q9EP73 Mouse Programmed cell death 1 ligand 1

Gene Symbol CD274
Gene Full Name CD274 molecule
Background This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. [UniProt]
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  • ARG81930 Mouse CD274 / PD-L1 ELISA Kit standard curve image

    ARG81930 Mouse CD274 / PD-L1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
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