ARG21961

Mouse IgG2a Isotype Control antibody [HOPC-1]

Mouse IgG2a Isotype Control antibody [HOPC-1] for ELISA,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot

Overview

Product Description

Mouse Monoclonal antibody [HOPC-1] as a negative control antibody for Mouse IgG2a

Tested Application ELISA, FACS, FLISA, ICC/IF, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone HOPC-1
Isotype IgG2a, lambda
Target Name Mouse IgG2a
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1 - 5 ug/ml
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1] ELISA image

    ELISA: The plate was coated with serially diluted ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1]. Immunoglobulin was detected with ARG21887 Goat anti-Mouse IgG1 antibody (Biotin) (pre-adsorbed), ARG23806 Goat anti-Mouse IgG2a antibody (Biotin) (pre-adsorbed), ARG21895 Goat anti-Mouse IgG2b antibody (Biotin) (pre-adsorbed), ARG21902 Goat anti-Mouse IgG3 antibody (Biotin) (pre-adsorbed), ARG21545 Goat anti-Mouse IgA antibody (Biotin) (pre-adsorbed), and ARG21523 Goat anti-Mouse IgM antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP) and quantified.

  • ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1] control image

    Control: Paraffin-embedded Human gastric cancer tissue stained with ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1] (left) and ARG22191 anti-MMP9 antibody [SB15c] (right), followed by ARG23705 Goat anti-Mouse IgG2a (HRP) (pre-adsorbed), DAB and hematoxylin.

  • ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1] control image

    Control: Paraffin-embedded Human kidney cancer tissue stained with ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1] (left) and anti-MMP3 antibody (right) followed by ARG23705 Goat anti-Mouse IgG2a (HRP) (pre-adsorbed), DAB and hematoxylin.