ARG80206

Mouse TNF alpha ELISA Kit

Mouse TNF alpha ELISA Kit for ELISA and Mouse

Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit
publication_link Publications5

Component

Cat No Component Name Package Temp
ARG80206-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80206-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80206-003 Standard diluent buffer 20 ml 4°C
ARG80206-004 Antibody conjugate concentrate 400 µl 4°C
ARG80206-005 Antibody diluent buffer 16 ml 4°C
ARG80206-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80206-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80206-008 20X Wash buffer 50 ml 4°C
ARG80206-009 TMB substrate 12ml 4°C (Protect from light)
ARG80206-010 STOP solution 12ml 4°C
ARG80206-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80206 Mouse TNF alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TNF alpha in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Ms
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human Lymphotoxin alpha1/ beta2, Lymphotoxin alpha2/ beta1, TNF- alpha, TNF RI, TNF RII
Target Name TNF alpha
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name tumor necrosis factor
Alternate Names Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21926 Mouse TNF

Swiss-port # P06804 Mouse Tumor necrosis factor

Gene Symbol Tnf
Gene Full Name tumor necrosis factor
Background Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Images (1) Click the Picture to Zoom In

  • ARG80206 Mouse TNF alpha ELISA Kit standard curve image

    ARG80206 Mouse TNF alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice.

ELISA / Mouse

Sudirman S et al.
PLoS One.,  (2018)

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AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage.

ELISA / Mouse

Fang Cheng-Chieh et al.
Sci Rep.,  (2018)

publication_link

 

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Red Seaweed Eucheuma cottonii Ethanol Extract Regulates Inflammatory Mediators Production and Prevents Colonic Injury on Acute Colitis Disease in Mice

ELISA / Mouse

Sabri Sudirman et al.
Preprint.,  (2018)

publication_link

 

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In situ detecting changes in membrane lipid phenotypes of macrophages cultured in different cancer microenvironments using mass spectrometry.

ELISA / Mouse

Xu Yupin et al.
Analytica Chimica Acta,  (2018)

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Therapeutic effects of matrine derivate MASM in mice with collagen-induced arthritis and on fibroblast-like synoviocytes.

ELISA / Mouse

Yuming Zou et al.
Sci Rep. ,  (2017)

publication_link

 

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Title Download Link
ARG80206 Mouse TNF alpha ELISA Kit User's manual download icon Download