ARG30346

Myofibroblast / Fibrosis Antibody Panel

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] Rabbit mAb AGMK, Bov, Dog, Hu, Ms, Pig, Rat, Zfsh FACS, ICC/IF, IHC-P, WB 20 μl
ARG21965 anti-Collagen I antibody, pre-adsorbed Goat pAb Bov, Dog, Gpig, Hm, Hu, Ms, Pig, Rb, Rat, Sheep EM, ELISA, FLISA, FACS, ICC/IF, IHC-P, IHC-Fr, IP, WB 20 μg
ARG66162 anti-Fibronectin antibody Mouse mAb Hu, Ms, Rat IHC-P, WB 20 μg

Overview

Product Description Myofibroblasts are a differentiated cell type with a phenotype between a fibroblast and a smooth muscle cell. They play a physiological role in wound healing and fibrosis.

Myofibroblast/Fibrosis Antibody Panel is an all-in-one solution to make the research of myofibroblast and fibrosis easy and economical. This antibody panel comprises the myofibroblast markers including smooth muscle actin, collagen I, and fibronectin antibodies. It is the best solution to study myofibroblast and fibrosis in samples from human, mouse or rat.

Related news:
New antibody panels for Myofibroblasts and CAFs
Target Name Myofibroblast / Fibrosis

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (20) Click the Picture to Zoom In

  • ARG66162 anti-Fibronectin antibody WB image

    Western blot: 20 µg of Huh7 and Huh7 shHMGB1 cell lysates stained with ARG66162 anti-Fibronectin antibody (1:1000), ARG55914 anti-E-cadherin antibody (1:1000) and ARG65636 anti-HMGB1 antibody (1:2000).

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.

  • ARG21965 anti-Collagen I antibody, pre-adsorbed ICC/IF image

    Immunofluorescence: Human fibroblasts were stained with ARG21965 anti-Collagen I antibody (pre-adsorbed).

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

    Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.

  • ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-Fr image

    Immunohistochemistry: Frozen MC4R-KO Mouse liver section was stained with ARG21965 anti-Collagen I antibody (pre-adsorbed), anti-F4/80 antibody followed by secondary antibodies and DAPI.

  • ARG66162 anti-Fibronectin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG21965 anti-Collagen I antibody, pre-adsorbed WB image

    Western blot: Purified Human Type I Collagen stained with ARG21965 anti-Collagen I antibody (pre-adsorbed).

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin embedded Rat kidney section post uninephrectomy was stained with ARG21965 anti-Collagen I antibody (pre-adsorbed) followed by a secondary antibody and AEC.

  • ARG66162 anti-Fibronectin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.