ARG30006

NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin)

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG52312 anti-GFAP antibody Rabbit pAb Cat, Dog, Hu, Ms, Rat ICC/IF, IHC-P, IHC-Fr, IHC-FoFr , WB 50 μl
ARG52345 anti-Nestin antibody [4D11] Mouse mAb Hu, Ms, Rat ICC/IF, IHC-Fr, WB 50 μl

Overview

Product Description

Nestin is an intermediate filament protein expressed in dividing cells during the early development stages of nervous system and is utilized as a neural stem cell marker. The neural stem cells loss Nestin expression once the cell fate is determined. Following gliogenesis, Nestin is replaced by cell type-specific intermediate filaments, e.g., GFAP for astrocytes.

Glial fibrillary acidic protein (GFAP) is the major intermediate filament protein in mature astrocytes, a main type of glial cells in the central nervous system (CNS). GFAP is used as a marker to distinguish astrocytes from other glial cells during development.

arigo’s ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin) is excellent for distinguishing neural stem cells and mature astrocytes. Moreover, as GFAP is also expressed in adult neural progenitors. This antibody duo is also excellent for identification of adult neural progenitors in brain tissue.

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Images (6) Click the Picture to Zoom In

  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: Cultured neonatal Rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green.

    Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

  • ARG52312 anti-GFAP antibody ICC/IF image

    Immunofluorescence: Cultured neurons and glia stained with ARG52312 anti-GFAP antibody (red) and ARG52468 anti-Vimentin antibody (green) showing specific labeling of GFAP (red) and vimentin (green). Cells containing GFAP and vimentin appear yellow

  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG52345 anti-Nestin antibody [4D11] (red) at 1:500 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.

    The Nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.

  • ARG52312 anti-GFAP antibody WB image

    Western blot: Rat cortex lysate showing specific immunolabeling of the ~50 kDa GFAP protein stained with ARG52312 anti-GFAP antibody.

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western blot: Rat brain (P18) homogenate stained with ARG52345 anti-Nestin antibody [4D11].

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western blot: Rat brain (embryonic, E18), C6 and SH-SY5Y cell lysates stained with ARG52345 anti-Nestin antibody [4D11] (green) at 1:500 dilution.