NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin)
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
|Cat No||Component Name||Host clonality||Reactivity||Application||Package|
|ARG52312||anti-GFAP antibody||Rabbit pAb||Cat, Dog, Hu, Ms, Rat||ICC/IF, IHC-P, IHC-Fr, IHC-FoFr , WB||50 μl|
|ARG52345||anti-Nestin antibody [4D11]||Mouse mAb||Chk, Cow, Hrs, Hu, Ms, Pig, Rat||ICC/IF, IHC-Fr, WB||50 μl|
Nestin is an intermediate filament protein expressed in dividing cells during the early development stages of nervous system and is utilized as a neural stem cell marker. The neural stem cells loss Nestin expression once the cell fate is determined. Following gliogenesis, Nestin is replaced by cell type-specific intermediate filaments, e.g., GFAP for astrocytes.
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody|
Images (4) Click the Picture to Zoom In
ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: Cultured neonatal Rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green.
Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
ARG52312 anti-GFAP antibody ICC/IF image
Immunofluorescence: Cultured neurons and glia stained with ARG52312 anti-GFAP antibody (red) and ARG52468 anti-Vimentin antibody (green) showing specific labeling of GFAP (red) and vimentin (green). Cells containing GFAP and vimentin appear yellow
ARG52312 anti-GFAP antibody WB image
Western blot: Rat cortex lysate showing specific immunolabeling of the ~50 kDa GFAP protein stained with ARG52312 anti-GFAP antibody.
ARG52345 anti-Nestin antibody [4D11] WB image
Western blot: Rat brain (P18) homogenate stained with ARG52345 anti-Nestin antibody [4D11].